(a) Sequence alignment of the two isoforms of HsIFT46 with the CrIFT46 sequence. (*) completely conserved residues, (:) conservative substitution, and (.) somewhat conservative substitution that does not interchange hydrophobic and non‐hydrophobic residues. (b) GFP‐binder affinity pull‐down experiment of HsODA16 using GFP‐tagged HsIFT46 produced in HEK293 cells. Lanes: (1) 20% of HsODA16 input, (2) elution of GFP‐binder beads incubated with HsODA16 (negative control), (3) elution of GFP‐HsIFT46 Isoform 1 incubated with a 10× molar excess of HsODA16, and (4) elution of HsIFT46 Isoform 2 incubated with a 10× molar excess of HsODA16