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. 2020 May 15;16(5):e1008190. doi: 10.1371/journal.ppat.1008190

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© 2020 Yadav et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — a. Western blot analysis of whole cell lysates isolated from transfectant cdc45^-/-/+ promastigotes expressing Cdc45-FLAG or Cdc45-PIP-FLAG. b. Analysis of growth patterns of transfectant cdc45^-/-/+ promastigotes expressing Cdc45-FLAG or Cdc45-PIP-FLAG. Graph presents averages of three experiments with error bars indicating standard deviation. c. Flow cytometry analysis of transfectant cdc45^-/-/+ promastigotes expressing Cdc45-FLAG or Cdc45-PIP-FLAG. The experiment was performed thrice, and the data from one experiment is presented here. 30000 events were analyzed at every sampling. Sampling times are indicated along the y axes and cell types are indicated below the histogram columns. cdc45^+/+/+—Ld1S::neo-hyg-bleo cells.