Fig 1. Recombinant OspC_N40-D10/E9 binds to fibronectin and dermatan sulfate.

Quadruplicate wells were incubated with 10 μg/ml of heparan sulfate (Hep-SO₄), chondroitin-4-sulfate (Chon-4-SO₄), dermatan sulfate (DS), chondroitin-6-sulfate (Chon-6-SO₄), fibronectin (Fn), laminin (Ln), type I collagen (Collagen I), type IV collagen (Collagen IV) or BSA (see Materials and Methods). Two μM recombinant GST-tagged OspC_N40-D10/E9 (black bars) or GST (negative control; white bars) was then added to the wells. Bound protein was measured by ELISA and mean OD₄₀₅ ± SEM was determined. Asterisks indicate that binding of GST-OspC to dermatan sulfate or fibronectin was significantly (p ≤ 0.05) greater than binding by GST by Student’s t-test. Shown is a representative of three independently performed experiments.