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. 2020 May 15;16(5):e1008516. doi: 10.1371/journal.ppat.1008516

Table 1. OspC variants differ in binding to dermatan sulfate and fibronectin.

		ELISA	----- Surface Plasmon Resonance -----
OspC variant	Ligand	K_D (μM)	K_D (μM)	k_on (10⁴s^-1M^-1)	k_off (s^-1)
B. burgdorferi
OspC_N40-D10/E9	Derm S0₄	0.16±0.05	0.27±0.07^a	4.64±1.94^a	0.01±0.003^a
	Fibronectin	0.35±0.03	0.30±0.07^a	45.08±14.94^a	0.13±0.04^a
OspC_B31	Derm S0₄	0.40±0.07	0.57±0.17^a	1.71±0.17^a	0.01±0.004^a
	Fibronectin	3.46±1.46^b	9.21±4.04^a^,^b	1.99±0.70^a	0.13±0.05^a
OspC_B31-ECM-	Derm S0₄	n.b.^c	n.b.^c	n.b.^c	n.b.^c
	Fibronectin	2.14±1.82^b	n.d.^d	n.d.^d	n.d.^d
OspC₂₉₇	Derm S0₄	0.60±0.13	0.75±0.32^a	1.69±0.35^a	0.01±0.002^a
	Fibronectin	2.78±1.46^b	1.75±0.40^a^,^b	3.68±0.24^a	0.06±0.005^a
B. garinii
OspC_PBr	Derm S0₄	5.53±3.92^b	9.97±2.19^a^,^b	0.040±0.001^a	0.003±0.001^a
	Fibronectin	0.90±0.20	0.71±0.08^a	19.20±5.95^a	0.13±0.04^a
B. afzelii
OspC_VS461	Derm S0₄	2.01±0.31^b	4.21±1.10^a^,^b	0.042±0.002^a	0.002±0.001^a
	Fibronectin	0.52±0.08	0.80±0.34^a	15.06±3.94^a	0.09±0.03^a

All values represent the mean ± SEM of three experiments.

^aThe K_D values were obtained from the average k_off divided k_on from each run.

^bThe values should be considered estimates due to the weakness of the interaction.

^cNo binding activity was detected.

^dNot determined.