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. 2020 May 15;16(5):e1008572. doi: 10.1371/journal.ppat.1008572

Fig 3. Both cps1-1 and cps1-1:Δgra24 tachyzoites require supplemental uracil for sustained replication.

Fig 3

Tachyzoites of strains cps1-1 or cps1-1:Δgra24 were inoculated onto human foreskin fibroblast monolayers in the presence (A and C) or absence (B and D) of exogenous uracil. Two days after infection, monolayers were fixed and stained with FITC conjugated anti-Toxoplasma antibody (green), phalloidin-Texas red to stain host cell actin, and DAPI (blue) to stain nuclei. Images were collected under 40x magnification. (E) Percent infection and the average parasites per vacuole were quantified in cps1-1 and cps1-1:Δgra24 infected fibroblasts in the presence and absence of exogenous uracil. This experiment was repeated twice with the same result. Data shown are the mean counts of three independent experiments. Statistical significance was assessed using Mann-Whitney test comparing cps1-1 (+/-) exogenous uracil and cps1-1:Δgra24 (+/-) exogenous uracil (E) (*** p < 0.001).