Flow chart of the order of 7 different testing conditions in (a) porcine and (b) human donor eyes, to evaluate the influence of baseline IOP, OPA, and frequency. The parameters were varied one at a time from nominal conditions (i.e., base = 15 mm Hg, OPA = 3 mm Hg, frequency = 1.2 Hz) to cover the normal ranges seen in humans. In porcine eyes, the nominal condition was performed at the beginning (nom1) and repeated once at the end (nom2), while in human donor eyes the nominal condition was performed at the beginning (nom1) and repeated after each group of testing (nom2, nom3, nom4). Base, baseline IOP; freq, simulated ocular pulse frequency; OPA, simulated OPA.