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Primary mouse hepatocytes were isolated from mice and treated with either DMSO vehicle or the TGF-β receptor antagonist, SB-431542. The cells were then exposed to room air or 1% oxygen for 72 hours and mRNA levels of Cxcl12 were measured by real-time PCR. n=4 where each n represents cells isolated from different mice. aSignificantly different from cells cultured in room air. bSignificantly different from DMSO-treated cells cultured in 1% oxygen.
