Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain backgroundMus musculus	C57Bl/6J	Jackson Labs or in-house colony	Stock: 000664
Recombinant DNA reagent	AAV5-GFAP-iGluSnFr	Addgene/University of Pennsylvania Vector Core	Addgene: 98930-AAV5 Penn: AV-5-PV2723
Recombinant DNA reagent	AAV5-GfaABC1D-tdtomato	Addgene/University of Pennsylvania Vector Core	Addgene: 44332-AAV5 Penn: AV-5-PV3106
Recombinant DNA reagent	AAV1-hSyn-EGFP	Addgene	Cat# 50465-AAV1
Recombinant DNA reagent	AAV1-hSyn-iGluSnFr	Addgene	Cat# 98929-AAV1
Chemical compound, drug	Sulforhodamine 101 (SR-101)	Sigma	Cat# S7635-50MG	Aqueous Stock: 0.5 mM Working: 0.5 µM
Chemical compound, drug	DNQX	Tocris	Cat# 189	DMSO Stock: 20 mM Working: 20 µM
Chemical compound, drug	AP5	Abcam	Cat# ab120003	Aqueous Stock: 50 mM Working: 50 µM
Chemical compound, drug	Gabazine/SR95531	Tocris	Cat# 1262	Aqueous Stock:10 mM Working: 10 µM