Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Mus musculus) | C2C12 | ATCC | CRL-1772 | |
| Cell line (M. musculus) | Ndufa9-knockout C2C12 | PMID:27622560 | ||
| Cell line (M. musculus) | Primary mouse embryonic fibroblasts (MEF) | Lonza | M-FB-481 | |
| Cell line (Homo sapiens) | Primary human skeletal myoblasts (HSkM) | Thermo Fisher Scientific | A11440 | |
| Recombinant DNA reagent | Doxycycline-inducible luciferase (pLVX-TRE3G-Luc) | Takara Bio (formerly Clontech) | Sold as part of 631187 | |
| Recombinant DNA reagent | Doxycycline-inducible LbNOX | PMID:27124460 | Available without inducible system on Addgene (#75285) | |
| Recombinant DNA reagent | Doxycycline-inducible mitoLbNOX | PMID:27124460 | Available without inducible system on Addgene (#74448) | |
| Recombinant DNA reagent | Doxycycline-inducible Saccharomyces cerevisiae NDI1 | PMID:27124460 | ||
| Other | Human adenovirus type5 expressing eGFP | Vector Biolabs | 1060-HT | |
| Other | Human adenovirus type5 expressing eGFP and LbNOX | Vector Biolabs (PMID:27124460) |
||
| Other | Human adenovirus type5 expressing eGFP and mitoLbNOX | Vector Biolabs (PMID:27124460) |
||
| Other | Dulbecco’s Modified Eagle’s Medium (DMEM), 4 mM L-Glutamine, no glucose, no sodium pyruvate | Thermo Fisher Scientific | 11966–025 | |
| Other | Dialyzed fetal bovine serum (dFBS) | Thermo Fisher Scientific | 26400–044 | |
| Other | Dialyzed horse serum | Valley Biomedical | AS3053-DI | |
| Other | Horse serum, New Zealand origin | Thermo Fisher Scientific | 16050–130 | |
| Chemical compound, drug | D-(+)-Glucose solution | Sigma | G8769 | Working concentration 10 mM |
| Chemical compound, drug | Doxycycline hyclate | Sigma | D9891 | 300 ng/ml |
| Chemical compound, drug | Piericidin A | Santa Cruz | Sc-202287 | 0.5 μM |
| Chemical compound, drug | Antimycin A | Sigma | A8674 | 0.5 μM |
| Chemical compound, drug | Oligomycin A | Sigma | 75351 | 1 μM |
| Chemical compound, drug | BAM15 | TimTec | ST056388 | 0.5 μM (or as indicated) |
| Chemical compound, drug | Chloramphenicol | Sigma | C0378 | 20 μg/ml |
| Chemical compound, drug | Tunicamycin | Sigma | T7765 | 1 μg/ml |
| Chemical compound, drug | L-Aspartic acid | Sigma | A9256 | 10 mM |
| Chemical compound, drug | L-Asparagine monohydrate | Sigma | A8381 | 0.5 mM |
| Chemical compound, drug | Sodium pyruvate solution | Thermo Fisher Scientific | 11360–070 | 1 mM |
| Chemical compound, drug | Uridine | Sigma | U3003 | 200 μM |
| Chemical compound, drug | GCN2iB | Acme Bioscience (PMID:30061420) |
0.5 μM | |
| Chemical compound, drug | GSK2656157 | Cayman Chemical | 17372 | 0.25 μM |
| Chemical compound, drug | Sodium DL-Lactate-3,3,3-d3 | CDN Isotopes | D6556 | |
| Chemical compound, drug | Sodium pyruvate-13C3 | Sigma | 490717 | |
| Chemical compound, drug | Adenosine-15N5 5′-Monophosphate | Sigma | 900382 | |
| Chemical compound, drug | Adenosine-13C5 5’-Diphosphate | Toronto Research Chemicals | A281697 | |
| Chemical compound, drug | Adenosine-13C10 5′-Triphosphate | Sigma | 710695 | |
| Chemical compound, drug | L-Aspartic acid-1,4-13C2 | Cambridge Isotope Laboratories | CLM-4455 | |
| Chemical compound, drug | Formic acid, LC/MS grade | Thermo Fisher Scientific | A117-50 | |
| Chemical compound, drug | Ammonium hydrogen carbonate, LC/MS grade | Sigma | 5.33005 | |
| Commercial assay or kit | TaqMan gene expression master mix | Thermo Fisher Scientific | 4369514 | |
| Commercial assay or kit | Ubr3 mouse TaqMan probe | Thermo Fisher Scientific | Mm01328979_m1 | |
| Commercial assay or kit | Atf3 mouse TaqMan probe | Thermo Fisher Scientific | Mm00476033_m1 | |
| Commercial assay or kit | Ddit3 mouse TaqMan probe | Thermo Fisher Scientific | Mm01135937_g1 | |
| Commercial assay or kit | Atf4 mouse TaqMan probe | Thermo Fisher Scientific | Mm00515325_g1 | |
| Commercial assay or kit | Gdf15 mouse TaqMan probe | Thermo Fisher Scientific | Mm00442228_m1 | |
| Commercial assay or kit | Ppp1r15a mouse TaqMan probe | Thermo Fisher Scientific | Mm01205601_g1 | |
| Commercial assay or kit | Trp53inp1 mouse TaqMan probe | Thermo Fisher Scientific | Mm00458142_g1 | |
| Commercial assay or kit | TBP human TaqMan probe | Thermo Fisher Scientific | Hs00427620_m1 | |
| Commercial assay or kit | GDF15 human TaqMan probe | Thermo Fisher Scientific | Hs00171132_m1 | |
| Antibody | GCN2 (rabbit, polyclonal) | Cell Signaling Technology | 3302 | 1:500 dilution |
| Antibody | Phospho-GCN2 (Thr899) (rabbit, monoclonal) | Abcam | ab75836 | 1:500 dilution |
| Antibody | ATF4 (rabbit, monoclonal) | Cell Signaling Technology | 11815 | 1:500 dilution |
| Antibody | eIF2α (mouse, monoclonal) | Cell Signaling Technology | 2103 | 1:500 dilution |
| Antibody | Phospho-eIF2α (Ser51) (rabbit, monoclonal) | Cell Signaling Technology | 3597 | 1:500 dilution |
| Antibody | Actin (mouse, monoclonal) | Sigma | A4700 | 1:3000 dilution |