Figure 1.

(A) Schematic of tetra-primer ARMS-PCR with lin-65(n3441)SNP indicated in white, WT SNP in yellow, and mismatches in red. (B) Three replicates of single-worm PCR products of C. elegansgene lin-65in N2 strain, a strain homozygous for the lin-65(n3441)SNP allele, and heterozygous (n3441/WT) F₁progeny under standard PCR conditions (left) and conditions optimized for amplification of the SNP flanking sequence (right). (C) PCR products of parental, F_­1, and F₂individuals with flanking sequence amplification.