FIG. 2.

Functional maintenance of the hPSC-HEP monolayer and constrained spheroids cell culture. hPSC-HEPs grows to a monolayer on the non-modified surface and form spheroids on the PEG-galactose-modified surface. hPSC-HEPs were cultured for 72 h and assessed for their hepatocyte-specific functions. (a) Phase-contrast images show that of hPSC-HEPs can remain attached under perfusion for both monolayer and spheroid configurations. Scale bar represents 100 μm. (b) Albumin secretion; (c) Urea synthesis; and (d) CYPs gene expression. All functional data were normalized to the DNA content in samples. Data are presented as means ± SEM of three biological replicates.