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. 2020 May 27;91(5):053703. doi: 10.1063/1.5144487

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© 2020 Author(s).

0034-6748/2020/91(5)/053703/14/$0.00

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FIG. 8. — Cardiac light-sheet imaging. Single-plane SPIM recording of the beating heart in a live 5-dpf larval zebrafish with the endocardium fluorescently labeled (GFP), showing six distinct time points during the cardiac beating cycle. These subcellular 2D images are comparable to our previous efforts²¹ as well as recent work by others.³¹ A false-color (fire) lookup table was used to enhance visualization. Frames were captured with a magnification of 11× and 5 ms exposure time at a rate of 85 frames/s. Figure 9 (Multimedia view) shows a movie of the same data. Scale bar: 50 µm.