TABLE I.

Flex-SPIM functional subsystems and modules. CW—continuous-wave, polarization beamsplitting optics—half-wave plate and polarizing beamsplitter, AOTF—acousto-optic tunable filter, galvo—galvanometer, and LED—light-emitting diode.

Subsystem/module	Description	Main componentsa
Multi-laser	Laser modulation and beam routing	CW lasers, beam expander, dichroic mirrors, polarization beamsplitting optics, AOTFs, and mirrors Ultrafast laser, polarization beamsplitting optics, beam expander, Pockels cells, and mirrors
Illumination-scanning optics	Generating the light-sheet	Scanning galvo mirrors and optics, mirrors, and objectives
Detection	Image capture	Scientific camera, tube lens, filter wheel, filters, and objective
Sample mounting and motion control	Holding and imaging the 3D sample	Custom sample chamber/holder, motion stages, and piezoelectric z-stage
Instrument control	Control and timing of components	Acquisition computer, national instruments PXI, system mainframe and scaling amplifiers
Auxiliary	Illumination masks and view-finding	Camera, LED, filter, and aluminum masks

^aThe main parts for each subsystem and module used to build the flex-SPIM are listed in Table SI.