Figure 4. Display of clusters of rare cell types in GQTSOM-based embedding.

Top left: Overview of the cleaned and embedded Unen dataset, colored by expression of main cell lineage markers. The contour based on Gaussian difference is added for easier identification of changes in cell density. Labels mark the rare cell types identified by van Unen et al. ¹⁵, Belkina et al. ¹⁶: (a) CD4 ⁺CD28 ^–CCR7 ⁺, (b) CD4 ⁺CD28 ^–CCR7 ^–CD56 ^–, (c) CD4 ⁺CD28 ^–CCR7 ^–CD56 ⁺, and (d) CD7 ⁺CD3 ^–CD127 ^–CD45RA ^–CD56 ^partial. Top right: Expressions of separate markers used for the identification. Bottom: Cells color-coded by sample origin (left) and separated by disease status of the patient (right).