. Author manuscript; available in PMC: 2020 Jun 1.

Published in final edited form as: Curr Osteoporos Rep. 2020 Jun;18(3):189–198. doi: 10.1007/s11914-020-00572-9

Table 1.

Cell lineage of potential SSCs in physiological conditions

Gene	Constitutively active cre or inducible creER	Tamoxifen injection	Chase	Cell lineage
Prrx^{23, 24}	Cre	–	E16.5	All cells derived from limb bud mesoderm
Prrx^{23, 24}	Cre	–	Adult	All skeletal cells in the limbs, including SSCs
Col2a1²⁷	Cre	–	P3	Chondrocytes, perichondrial cells, osteoblasts, and bone marrow stromal cells
	CreER	E13.5	E14.5	Chondrocytes and perichondrial cells
		E13.5	P21	Cells in the growth plate, perichondrium, bone, and bone marrow
		P3	P5	Columnar chondrocytes and borderline chondrocytes in the growth plate, a small number of cells in the perichondrium and the metaphyseal spongiosa
		P3	1Y	Chondrocytes, osteoblasts, stromal cells, and adipocytes in the metaphyseal bone
Pthrp²⁹	CreER	P6	P12	A subset of the resting chondrocytes (growth plates SSCs)
Pthrp²⁹	CreER	P6	1Y	Columnar chondrocytes in the growth plate, osteoblasts, and bone marrow stromal cells
Cathepsin K³¹	Cre	–	E14.5	Cells in the perichondrium
Cathepsin K³¹	Cre	–	P10	Cells in the periosteal mesenchyme and the endosteal marrow component, as osteoclasts
Osterix^{42, 52}	CreER	E13.5	E14.5	Perichondrial cells in the osteogenic perichondrium, and proliferating chondrocytes in the cartilage template
			E16.5	Cells in the trabecular bone and bone marrow stromal cells, not in the periosteum
			13W	Cells decreased in the metaphyseal marrow space
		P5	P6	Cells in the primary spongiosa and around the cortical bone
		P5	24W	Osteoblasts, osteocytes, and bone marrow stromal cells in the metaphyseal marrow space
αSMA⁴⁹	CreER	4–5W	2D chase	Cells in the primary spongiosa and within the periosteum
αSMA⁴⁹	CreER	4–5W	17D chase	Some osteoblasts and osteocytes in the marrow space
Lepr⁵³	Cre	–	P0.5	Bone marrow stromal cells mostly in metaphysis
			2M	Bone marrow stromal cells in metaphysis and diaphysis
			14M	Bone marrow stromal cells, osteoblasts, osteocytes, and adipocytes throughout the marrow space
Ebf3⁶⁰	CreER	10W(×4)	2D chase	Bone marrow stromal cells exclusive of osteoblasts
Ebf3⁶⁰	CreER	10W(×4)	13M chase	Bone marrow stromal cells, osteoblasts, and adipocytes
Cxcl12³²	CreER	P3	P5	Bone marrow stromal cells in the central marrow space
		P3	1Y	Bone marrow stromal cells and some trabecular osteoblasts in far proximal portion of the marrow space
		8W	2D chase	Bone marrow stromal cells in the central marrow space
		8W	1Y	Bone marrow stromal cells and some trabecular osteoblasts in the central marrow space