Fig. 2. The effects of ACOT7 silencing on the proliferation, cell cycle progression, apoptosis, migration, and invasion of SGC7901 cells. (A) The expression of ACOT7-1 and ACOT7-2 after transfection under a fluorescence microscope using GFP (×200). (B) The expression of ACOT7-1 and ACOT7-2 proteins after transfection upon Western blot assay and PCR. (C) The proliferation rate of SGC7901 after transfection. (D) Cell cycle progression of SGC7901 after transfection. (E) Flow cytometry analysis of apoptosis of SGC7901 cells after transfection by flow cytometry. (F) Cell migration and invasion of SGC7901 cells after transfection using crystal violet staining (×200). (G) The scratch test was performed by scratching a confluent monolayer of cells with a 200-uL pipette tip. The migration of SGC7901 cells was analyzed after 24 hours. ^***Compared with empty vector, p<0.001. GFP, green fluorescent protein; ACOT7, acyl-CoA thioesterase 7.