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. Author manuscript; available in PMC: 2020 May 29.
Published in final edited form as: Plant Cell Environ. 2015 Dec 14;39(3):645–651. doi: 10.1111/pce.12655

Figure 5. ASR1 binds to the STAR1 promoter in vitro in a non-preferential manner.

Figure 5.

(A) SAPA pull-down system showing that ASR1-GST binds to the STAR1 DNA biotinylated fragment. ASR5 was used as competitor. (B) SAPA pull-down system showing that ASR5 binds preferentially to the STAR1 DNA biotinylated fragment. ASR1-GST was used as competitor. GST alone was used as a negative control. (C) Transient gene expression assays showing regulation of STAR1 by ASR1 using GUS/Luciferase assay. Values represent the means +/− SD (N=4). Asterisks indicate significantly different means: (*) P<0.05.