Fig. 2.

The efficacy of mAb785 as ligand mediating the targeting of NPs to multiple breast cancers. a The targeting of mAb785 to four genotypes of breast cancer cells. b Preparation and SEM image of mAb785-NPs, confirmation of the successful conjugation of mAbs onto the NPs by flow cytometry, and the determination of targeting capacity of mAb785-NPs to multiple breast cancer cells by fluorescent microscope (Bar = 100 μm, Two-tailed Student's t test was performed for statistical analysis). c Multiple breast cancer cells (ZR75.1, MCF-7, SKBR3, and MDA-MB-231) were cocultured with non-breast cancer cells (control cells, GFP positive). mAb-NP-Dox (Red fluorescence) was incubated with cocultured cells and the relative red fluorescent intensities were measured along the white arrowheads (Bar = 50 μm). d intensity distribution of red and green fluorescence which indicated the distribution of red fluorescent signals were opposite to that of green fluorescent signals, that was, GFP+ cells were detected with relative low red fluorescence while GFP− cells were detected with relative high red fluorescence (two-tailed Student's t test was performed for statistical analysis)