Fig. 5.

Location of mAb785 targeted epitope determination and computer-assisted structure reconstruction. a Three enriched phages were used for the pre-blocking test. Only phage 733 (P733) could completely block mAb785 immunostaining in breast cancer sections (Bar = 100 μm). b The three variant amino acids in peptides carried by phage 733 were highly similar to those in the Mam-A protein. c Replacing the three variant amino acids in the peptides from phage733 did not influence their reactivities with mAb785 (Bar = 100 μm). d Removing the last two amino acids in the peptides did not influence their reactivities with mAb785. e Removing the first two amino acids in the peptides significantly influenced their reactivities with mAb785. f The epitope for mAb785 in Mam-A was determined as the N-terminal 42–51 amino acids