Table 2.
EC50 values for OR2M3 with amino acid changes at positions of a putative copper/odorant-binding pocket measured in the absence and presence of 10 µmol/L Cu2+
Receptor | Agonist | EC50 in the absence of Cu2+ in µmol/La | EC50 in the presence of Cu2+ in µmol/La |
---|---|---|---|
OR2M3 T105A | 3-Mercapto-2-methylpentan-1-ol | 1.42 ± 0.58 | 0.75 ± 0.27 |
OR2M3 C112A | 3-Mercapto-2-methylpentan-1-ol | n.d. | 0.86 ± 0.004 |
OR2M3 C203S | 3-Mercapto-2-methylpentan-1-ol | n.d. | 3.16 ± 0.01 |
n.d. no detectable response up to 100 µmol/L
aMean ± SD (n = 3–5)