Table 3.

EC₅₀ values for OR2W1 with amino acid changes at positions of a putative copper/odorant-binding pocket measured in the absence and presence of 10 µmol/L Cu²⁺

Receptor	Agonist	EC50 in the absence of Cu2+ in µmol/La	EC50 in the presence of Cu2+ in µmol/La
OR2W1 L202S	2-Phenylethanethiol	97.03 ± 13.01	96.73 ± 4.66
OR2W1 L202C	2-Phenylethanethiol	65.08 ± 10.80	63.41 ± 3.77
OR2W1 S109A	2-Phenylethanethiol	34.41 ± 9.98	38.93 ± 5.38
OR2W1 L202S	3-Mercaptohexyl acetate	146.00 ± 6.48	310.73 ± 22.11
OR2W1 L202C	3-Mercaptohexyl acetate	98.89 ± 7.49	114.43 ± 10.46
OR2W1 S109A	3-Mercaptohexyl acetate	146.49 ± 23.01	126.95 ± 84.89
OR2W1 L202S	Allyl phenyl acetate	37.60 ± 11.68	46.00 ± 14.34
OR2W1 L202C	Allyl phenyl acetate	129.92 ± 82.50	73.00 ± 42.12
OR2W1 S109A	Allyl phenyl acetate	46.04 ± 11.63	118.90 ± 19.92

n.d. no detectable response up to 100 µmol/L

^aMean ± SD (n = 3–5)