Table 2.
Characterization of PA tissue samples and pituispheres for hormone and cell marker expression by immunohistochemistry (IHC) and immunofluorescent analysis (IF).
| Analysis | Marker | PA01 | PA02 | PA03 | PA04 | PA05 |
|---|---|---|---|---|---|---|
| IHC of paraffin-embedded PA tissue | GH | 3 | 2 | 0 | 0 | |
| PRL | 3 | 1 | 1 | 0 | ||
| ACTH | 1 | 2 | 0 | 1 | ||
| TSHb | 0 | 0 | 1 | 0 | ||
| LHb | 0 | 1 | 1 | 0 | ||
| FSHb | 0 | 1 | 0 | 0 | ||
| CGA | 1 | 3 | 3 | 2 | ||
| SSTR2 | 0 | 2 | 2 | 3 | ||
| SSTR5 | 1 | 2 | 1 | 3 | ||
| AIP | 2 | 3 | 2 | 3 | ||
| CK8 densely granulated | 2 | 2 | 1 | 1 | ||
| CK8 sparsely granulated | 2 | 3 | 0 | 0 | ||
| Pit-1 | 3 | 3 | 2 | 1 | 1 | |
| SF-1 | 0 | 2 | 1 | 3 | 2 | |
| Tpit | 0 | 1 | 1 | 0 | 1 | |
| CD15 | 1 | 1 | 1 | |||
| IF characterization of pituishperes | GFRa2 | + | + | – | + | |
| Pit-1 | + | + | + | – | ||
| CD15 | + | + | + | + | ||
| SF-1 | + | + | – | + | ||
| NES | – | – | – | – | ||
| SOX2 | – | – | – | – |
IHC protein expression evaluation was performed using 0–3-mark system with 1 = <30% positive cells; 2 = 30–70% positive cells; 3 = >70% positive cells, IF protein expression positive (+) if at least a few cells in the sphere were positive, negative (–) if not a single cell in a sphere expressed the marker, blank boxes: not done.