Fig. 1.

Construction of recombinant baculovirus, Bac-EGP64. A-a Schematic representation of ZIKV polyprotein. Envelop (E) protein (aa. 1–504, green). A-b The strategy of fusion proteins. Recombinant EGP64 contained GP64 SP, ZIKV E, GP64 TM. A-c Representation of ZIKV E protein recombinant expression constructs. The E protein coding sequence was inserted between the signal peptide (SP) and transmembrane domain (TM) of AcMNPV bacmid envelope protein GP64 under the control of a polyhedrin promoter (PH), to ensure proper expression and transportation. B The perceived budding process of Bac-EGP64. The fusion protein EGP64 was displayed on the surface of Sf9 cell membrane and on the envelope of baculovirus following budding. C Flow chart of the construction process from cloning of ZIKV E gene into the pFastBac to the production of recombinant baculovirus.