Fig. 5.

Protective efficacy of Bac-EGP64 in AG6 mice challenged by ZIKV. A AG6 mice (n = 6) were immunized with three doses of EG, WT or PBS at weeks 0, 2 and 4, then challenged by ZIKV at week 6 and blood samples were collected at day 3 after challenge. The challenged mice were monitored daily for 28 days for weight, clinical scores, viremia and survival. B Weight of the challenged mice. C Viremia detection of AG6 mice at day 3 after challenge. D Survival curves of challenged AG6 mice. Representative data of two independent experiments are shown. E Clinical scores of the challenged mice. Clinical scores were graded as follows: 0, healthy; 1, retardation and reduced mobility; 2, limb weakness; 3, tremors or imbalance; 4, paralysis; and 5, moribund or dead. ***P < 0.001.