Figure 2.

Perforin deficiency aggravates HFD-induced liver injury and steatohepatitis. WT and Prf^−/− mice were fed SFD or HFD for 10 weeks at 6–8 weeks of age. (A) Body weight (left panel) and liver weights (right panel) (n = 4–5), (B) measurements of serum ALT (left panel) and cholesterol levels (right panel) (n = 5–10), and (C) measurements of liver TG content (n = 4–8). (D) Representative images of liver sections stained with H&E and Oil red O. (E) Expression levels of lipogenic genes (top) and fatty acid oxidation genes (bottom) in the liver, relative to Hprt expression levels (n = 4–9). The data shown in each panel of the figures were collected from a single experiment, and each experiment was repeated for at least three times and rendered consistent results. Means ± SEM, *p < 0.05; **p < 0.01, ***p < 0.001.