Figure 5.

Perforin regulates fatty liver disease through CD4 T cells in the liver. WT and Prf^−/− mice were injected with PBS, or Prf^−/− mice were injected with an anti-CD4 antibody weekly during 10 weeks of HFD challenge. (A) Body and liver weight were measured (n = 6–8). (B) Measurements of liver TG content (n = 4–7). (C) Representative images of liver sections stained with H&E (top) and Oil red O (bottom). (D) Serum TNF-α and IFN-γ levels were determined (n = 4–6). (E) Representative immunofluorescence images from liver with DAPI (blue), F4/80 (red), iNOS (green), and co-localization (merged image) (left) and the percentage of M1/M (refers to iNOS+F4/80+cells/F4/80+ cells) was calculated (right). (F) Expression levels of lipogenic genes (left) and fatty acid oxidation genes (right) in the liver relative to Hprt expression levels (n = 6–11). The data shown in each panel of the figures were collected from a single experiment, and each experiment was repeated for at least three times and rendered consistent results. Means ± SEM, *p < 0.05; **p < 0.01, ***p < 0.001.