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. 2020 May 22;11:846. doi: 10.3389/fimmu.2020.00846

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Copyright © 2020 Wang, Li, Zhu, Zhang, Zhang, Cao, Zhu, Shi, Hao, Wen, Liu, Yang and Yin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hepatic steatosis in Prf^−/− mice is dependent on IFN-γ mediated inflammation. Prf^−/− and IFN-γR^−/− and Prf^−/− mice were fed HFD for 10 weeks. (A) Body and liver weight were measured (n = 4–7). (B) Serum levels of ALT (left panel, n = 4–7), cholesterol (middle panel, n = 4–7), and liver TG (right panel) were measured (n = 12–13). (C) Representative images of liver sections stained with H&E (left) and Oil red O (right). (D) TNF-α, IL-6, and IFN-γ levels were detected in liver culture supernatants (n = 4). (E) Representative immunohistochemistry of DAPI (blue), F4/80 (red), iNOS (green), and co-localization (merged image) in liver, and the percentage of M1/M (refers to iNOS+F4/80+cells/ F4/80+ cells) was calculated (right). The data shown in each panel of the figures were collected from a single experiment, and each experiment was repeated for at least three times and rendered consistent results. Means ± SEM, *p < 0.05; **p < 0.01, ***p < 0.001.