Figure 7.

CD4 T cells in Prf^−/− mice have an intrinsically elevated ability of IFN-γ production. CD4 T cells (A) and CD8 T cells (B) from WT and Prf^−/− mouse spleens were cultured in media supplemented with GolgiStop in the presence or absence of plate-bound anti-CD3 plus anti-CD28 for 4–6 h and then stained for intracellular IFN-γ (left, percentage; right, cell number, n = 5–6). (C) Naive CD4+ T cells were sorted from the spleen of WT or KO mice and differentiated to Th1 cells for 3 days. The IFN-γ production in these cells was detected by flow cytometry. Representative FACS plots and total cell number of IFN-γ+CD4+T cells were shown. The data shown in each panel of the figures were collected from a single experiment, and each experiment was repeated for at least three times and rendered consistent results. Means ± SEM, *p < 0.05; **p < 0.01.