Figure 7.

Abnormal T regulatory cell responses under intra-amniotic inflammation are exaggerated with TNFα inhibition. (A) Automated clustering of on T cells from cytokine panel (n = 3 per group). (B) Populations were identified based on antibody intensity. (C) FoxP3 expression CD4 and CD8 T cells, FoxP3+ cells are outlined in black and quantified as a percent of all T cells. Kruskal–Wallis values are listed at the top of each graph. Post hoc P-values are listed as lines connecting respective comparisons. *P < 0.05. (D) Heatmap of MMIs of cytokines in FoxP3⁺ and FoxP3⁻ T cells. Comparisons are made between treatment groups within each subset of T cells. Significance was determined using Kruskal–Wallis comparisons. Comparisons listed on heatmaps are those with an overall Kruskal–Wallis P < 0.1 and are placed on the box of the group with the most different value from other treatments. (E) Summary of findings in this article. SynT, syncytiotrophoblast.