Figure 3.

Gene conversion and SCE in HCC and immortalized hepatocytes.

(a) Unique DSBs were introduced into cells by electroporation of DR-GFP substrate and pCBAs-I-SceI plasmids. Relative gene conversion efficiency was expressed as a function of green fluorescent signal from the repair of DSBs on DR-GFP by gene conversion. (b) Number of chromosomes and (c) SCE events by metaphase analyses in HCC or immortalized hepatocytes were assessed using ImageJ and analyzed by Prism 6. Asterisks (*) indicates significant data point (p<0.005) by Kruskal-Wallis test. d) Representative metaphases demonstrating “Harlequin” chromosomes in HCC but MIHA. Number of Harlequin chromosomes increased with camptothecin treatment.