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Solubility prediction profile score of (A) intact ACE2 showing most of the residues in the window are below 0 (cyan) and −1 (red), while very few residues are above 1 (blue), favors insoluble expression for intact ACE2. (B) a large number of residues are between −1 and 1 (cyan), while a window of 7 residues at C-terminal are above 1 (blue), favoring total solubility score above 0, hence soluble expression for tACE2.
