Figure 12.

Agomir of miR-411-3p Inhibited Silicosis in Mice Exposed to Silica

(A) H&E, Sirius red staining, and positive expression of α-SMA showed silicotic lesions induced by SiO₂ in the silica and the silica+agomir-NC groups. Treatment with agomir miR-411-3p could reduce silicotic lesions compared to the silica+agomir-NC group (scale bars, 50 μm). (B) The miR-411-3p expression was shown by in situ hybridization (scale bars, 100 μm). (C) The level of miR-411-3p was reduced in the silica and the silica+agomir-NC groups and was increased in the agomir miR-411-3p group. The mRNA levels of Col I, α-SMA, SRF, and MRTF-A were increased in the silica and the silica+agomir-NC groups and were decreased in the agomir miR-411-3p group. (D) The protein levels of Col I, α-SMA, SRF, and MRTF-A were increased in the silica and the silica+agomir-NC groups and were decreased in the agomir miR-411-3p group. (Data indicate mean ± SD; n = 6 independent experiments.)