Figure 3.

MRTF-A Participates in Myofibroblast Differentiation in Silicosis

(A) The co-expression of MRTF-A and α-SMA in lung of rats (scale bars, 100 μm). (B) Protein levels of SRF and MRTF-A were increased in silicotic rats. (C and D) The (C) mRNA levels and (D) protein levels of Col, α-SMA, SRF, and MRTF-A were increased in lung fibroblasts induced by TGF-β1. (E) The effective siRNA screened by western blotting. (F) siRNA-MRTF-A reduced the levels of MRTF-A, COL I, and α-SMA. (Data indicate mean ± SD; n = 3 independent experiments.)