Figure 9.

miR-411-3p Inhibited Mrtfa in Lung Fibroblasts Activated by TGF-β1

(A) The schematic diagrams of the luciferase reporter construction. WT and mutant (Mut) 3′ UTR of Mrtfa containing the binding site with miR-411-3p were inserted into pmir-GLO vector. (B) 293T cells were transfected with miR-411-3p mimics/miR-NC and the plasmid of Mrtfa-3′ UTR Mut/WT, and the relative luciferase activity was significantly suppressed by miR-411-3p mimics in the Mrtfa-3′ UTR WT group. (C) The increasing level of miR-411-3p by miR-411-3p mimics. (D and E) The mRNA levels of Col I, α-SMA, SRF, and MRTF-A in (D) lung fibroblasts and (E) TGF-β1-induced lung fibroblasts transfected with miR-411-3p mimic/inhibitor. (Data indicate mean ± SD; n = 3 independent experiments.)