Figure 1.

SI Mice with Higher miR-206 Levels Exhibit a Higher Level of Aggression

(A) The experimental design and procedures. Mice at P21 were randomly assigned to group housing (GH) or social isolation (SI) groups. 5 weeks later, the mice were subjected to five footshocks 30 min before the RI test. (B) SI mice showed a higher number of biting attacks toward intruders compared with GH mice. Unpaired Student’s t test, t₍₁₃₎ = 4.90, ∗∗∗p < 0.001 versus GH; n = 8 and 7 in GH and SI mice, respectively. (C) vHip tissues were dissected out for quantitative real-time PCR analysis. SI mice showed higher levels of miR-206 compared with GH mice. Unpaired Student’s t test, t₍₁₃₎ = 3.074, ∗∗p < 0.01 versus GH. (D) There was a significant positive correlation when the number of attacks was plotted against the levels of miR-206 in SI mice. R² = 0.62, ∗p < 0.05, n = 7 in SI mice. Data represent mean ± SEM.