Figure 2.

The miR-206 Antagomir Increases BDNF Protein Expression

(A) AM206 had no effect on Bdnf mRNA in GH mice but increased Bdnf mRNA in SI mice. Two-way ANOVA, F_(1,22) = 9.18, ∗∗p < 0.01 in the interaction effect, Bonferroni’s post hoc test, scramble versus AM206, ∗∗p< 0.01 in SI mice, p = 0.72 in GH mice. n = 10, 5, 6, and 5 in scramble-GH, AM206-GH, scramble-SI, and AM206-SI mice, respectively. (B) The representative images of Western blotting in (C). (C) AM206 significantly increased the protein expression of miR-206 downstream targets. Two-way ANOVA, significant main effects of AM206: F_(1,22) = 9.72, ∗∗p < 0.01 in BDNF protein levels; F_(1,22) = 118.6, ∗∗p < 0.001 in proBDNF protein levels; F_(1,22) = 180.2, ∗∗p < 0.001 in p-TrkB levels; F_(1,22) = 18.6, ∗∗p < 0.001 in HDAC4 protein levels. In scramble-treated mice, BDNF, proBDNF, and p-TrkB protein levels were significantly lower in SI mice compared with GH mice. GH versus SI, t₍₁₄₎ = 9.55, #p< 0.05, t₍₁₄₎ = 6.34, ##p< 0.01, and t₍₁₁₎ = 3.46, ##p< 0.01 in BDNF, proBDNF, and p-TrkB of scramble control, respectively. However, the HDAC4 level of scramble-treated GH mice was not different from the one of scramble-treated SI mice (t₍₁₁₎ = 0.30, p = 0.77). In total TrkB and CRF protein expression, there was no difference among groups (one-way ANOVA, F_(3,22) = 2.9, p > 0.05 and F_(3,22) = 0.14, p > 0.05 in TrkB and CRF, respectively). For BDNF and proBDNF protein, n = 10, 5, 6, and 5 in scramble-GH, AM206-GH, scramble-SI, and AM206-SI mice, respectively. For p-TrkB, TrkB, HDAC4, and CRF protein, n = 6, 7, 7, and 6 in scramble-GH, AM206-GH, scramble-SI, and AM206-SI mice, respectively. Data represent mean ± SEM.