Figure 3.

Distribution of Cy3-AM206 after Intra-hippocampal Injection

(A) The infusion site of Cy3-labeled AM206. The scale bar represents 550 μm. (B) The fluorescence of Cy3-AM206 was distributed throughout the vHip and present in NeuN-positive (top) and glial fibrillary acidic protein (GFAP)-positive (bottom) cells. The scale bar represents 50 μm. In the insets on the right, the scale bar represents 20 μm. (C) The percentage of Cy3⁺-NeuN⁺ cells among NeuN⁺ cells is higher than that of Cy3⁺-GFAP⁺ cells among GFAP⁺ cells. Unpaired Student’s t test, t₍₁₄₎ = 14.47, ∗∗∗p < 0.001. n = 7 and 9 brain slices in 3 mice for NeuN and GFAP, respectively. (D) BDNF expression (green) was highly colocalized with Cy3-positive cells (red) in the vHip of AM206-treated mice (bottom). The scale bar represents 50 μm. In the insets on the right, the scale bar represents 20 μm. (E) AM206 treatment increased BDNF protein expression in Cy3-positive cells compared with the scramble control. Unpaired Student’s t test, t₍₂₂₎ = 9.42, ∗∗∗p < 0.001. n = 12 brain slices in 3 mice per group. Data represent mean ± SEM.