Figure 4.

Intra-hippocampal Infusion of AM206 Produces Anti-aggression Effects in SI Mice

(A) In the RI test, there was a significant interaction effect in AM206 and SI mice (two-way ANOVA, F_(1,70) = 21.84, ∗∗∗p < 0.001). AM206-treated SI mice (500 μM, 0.8 μL/side) had significantly lower attack numbers than scramble-treated SI mice (500 μM, 0.8 μL/side) (Bonferroni’s post hoc test, p < 0.001). In contrast, intra-hippocampal AM206 infusion had no effect in GH mice (Bonferroni’s post hoc test, p > 0.1). For mismatch control of the AM206 sequence, mismatch-treated SI mice had significantly higher attack behavior than AM206-treated SI mice (one-way ANOVA, F_(2,46) = 16.47, ∗∗∗p < 0.001; Bonferroni’s post hoc test, p < 0.05), but mismatch-treated SI mice showed no difference from scramble-treated SI mice (Bonferroni’s post hoc test, p > 0.9). (B and C) AM206 had no effect on the locomotion activity (B) and central duration (C) in the open field test in GH and SI mice. Two-way ANOVA, F_(1,70) = 0.02, p = 0.89 in the interaction effect of total distance traveled (B). F_(1,70) = 0.22, p = 0.63 in the interaction effect of central duration (C). For mismatch control of the AM206 sequence, mismatch-treated SI mice showed no difference from AM206- and scramble-treated SI mice (one-way ANOVA, F_(2,46) = 0.59, p > 0.05 in total distance; F_(2,46) = 0.01, p > 0.05 in duration traveling in the central area). (D) In the FST, AM206 significantly reduced immobility time in SI mice but not in GH mice. Two-way ANOVA, F_(1,70) = 19.60, ∗∗∗p < 0.001 in the interaction effect of immobility duration. Bonferroni’s post hoc test, AM206 versus scramble, ∗∗p < 0.01 in SI mice. For mismatch control of the AM206 sequence, mismatch-treated SI mice showed no difference from scramble-treated SI mice (p > 0.9).n = 14 and 20 in scramble- and AM206-GH mice, respectively. n = 20, 20, and 9 in scramble-treated, AM206-treated, and mismatch SI mice, respectively. Data represent mean ± SEM.