Figure 5.

Knockdown of BDNF Prevents the Effects of AM206 in SI Mice

(A) Transduction with Bdnf shRNA into the vHip significantly decreased BDNF protein expression. Unpaired Student’s t test, t = 3.29, ∗p < 0.05, n = 3 per group. (B) The experimental procedure of combination of AM206 with BDNF knockdown. Six days after mice received a microinjection of AM206, the mice were transduced with a lentivirus carrying scramble or Bdnf shRNA through the cannula. Ten days later, the mice were given footshocks 30 min before the RI test. (C) BDNF knockdown increased the attack behavior of AM206-treated SI mice but did not have effects on AM206-treated GH mice. Two-way ANOVA, F_(1,28) = 26.63, ∗∗∗p < 0.001 in the interaction effect of the attack number. Bonferroni’s test, BDNF knockdown versus scramble, ∗∗∗p < 0.001 in AM206-treated SI mice, p > 0.05 in AM206-treated GH mice. n = 8 per group. (D) BDNF knockdown had no effects on total distance traveled in the open field test. Two-way ANOVA, F_(1,28) = 0.001, p > 0.05 in the interaction effect. n = 8 per group. All data represent mean ± SEM.