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. 2020 May 15;10:e00131. doi: 10.1016/j.mec.2020.e00131

Figure 5.

Figure 5

(A), (C–G): ANN glycosylation model fitting for three different glycosites in the Fc-DAO and EPO-Fc proteins. The performance of networks with two and three hidden layers was examined. (B): Expression of each b4GalT isoform in each engineered cell line with respect to the expression of the enzyme in the wild type (REF). The experimental data for A, C-G graphs are the average of the quadruple knockout b4GalT1/2/3/4 ​cell lines (D-1C3, D-1E1 and D-2E7). The experimental data for all graphs (A–G) are taken from Bydlinski et al. (2018). The glycans included in the graphs were present in the glycoform distributions of at least three of the triple knockout clones but were not detected in the quadruple knockout cell lines. Glycans measured in low abundances (<1%) in one or two knockout cell lines were not included in the analysis.