Fig. 1.

The filtration setup, MEW filter structure, and bioconjugation. (a) The MEW filter, marked with a white arrow, with diameter of Ø12 mm is transparent and can readily be mounted to standard filter holders. (b) The filtration setup consists of a syringe pump connected through a tubing to the filter holder and of a syringe attached on top of the filter holder, where the sample is loaded. (c) Scanning electron microscopy (SEM) image of the MEW filter. Scalebar, 300 μm. (d) The confocal microscopy image of MEW filter conjugated with a biotinylated anti-EpCAM antibody, which was detected by streptavidin alexa fluor 488 (green). The image was acquired, at 10% laser power. (e) A non-conjugated MEW filter was incubated with streptavidin alexa fluor 488 as a negative control. The laser power was set to 100%. MEW, melt electrowriting.