Figure 4. Inhibitory effect of DHE on hypoxia-induced HIF-1α expression in NSCLC cells.

A549 cells were treated with 0, 10, and 20 μM DHE for 24 h under normoxic or hypoxic condition. The mRNA and protein levels of HIF-1α in A549 cells were respectively determined using qRT-PCR (A) and Western blot (B). *P<0.05 vs. A549 cells under normoxic condition. ^#P<0.05 vs. A549 cells under hypoxic condition.