Table 5.
TF prediction of the hub DEGs.
| TF | NES | No. of targets | No. of motifs |
|---|---|---|---|
| Nf1 | 7.519 | 7 (C3, Cxcl2, Bub1b, Mapk3, Npy, Gnail, Agt) | 5 |
| Kdm2a | 7.270 | 3 (C3, Npy, Gnail) | 3 |
| Smad3 | 6.683 | 3 (C3, Npy, Agt) | 2 |
| Pbx3 | 6.324 | 3 (Npy, Gnail, Agt) | 5 |
| Rara | 6.269 | 6 (Cx3cr1, Bub1b, Mapk3, Npy, Gnail, Agt) | 9 |
| Neurod1 | 6.069 | 5 (Bub1b, Cst3, Npy, Gnail, Agt) | 3 |
| Gfi1b | 5.475 | 4 (C3, Bub1b, Npy, Agt) | 2 |
| Hoxa9 | 5.392 | 3 (Cxcl2, Bub1b, Gnail) | 2 |
| Esr2 | 5.392 | 3 (C3, Gnail, Agt) | 4 |
| Arid5a | 5.364 | 7 (Cxcl2, Bub1b, Cst3, Mapk3, Npy, Gnail, Agt) | 6 |
| Plagl1 | 5.316 | 3 (Bub1b, Npy, Agt) | 2 |
TF prediction of the hub DEGs was performed using the iRegulon plugin in Cytoscape with the criteria for motif enrichment analysis as follows: identity between orthologous genes ≥ 0.05, FDR on motif similarity ≤ 0.001, and normalized enrichment score (NES) > 5. Eleven TFs (Nf1, Kdm2a, Smad3, Pbx3, Rara, Neurod1, Gfi1b, Hoxa9, Esr2, Arid5a, and Plagl1) were predicted to regulate 9 of the 13 hub DEGs.