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MIC determination of polyP3 by the agar dilution method. The bacterial cells were spot-inoculated (approximately 105 to 106 cells/spot) onto Brucella blood agar plates containing polyP3 at various concentrations and incubated at 37°C for 3 days anaerobically. The MIC was defined as the lowest concentration that inhibited the bacterial growth on the plate. The results for P. intermedia ATCC 49046 are the same as those reported in our previous study (Jang et al. 2016).
