Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used for identification of L. monocytogenes.
| Primer Primer sequence (5' - 3') | Size of PCR product (bp) | Amplification conditions |
|---|---|---|
| L1 CAG CAG CCG CGG TAA TAC | 938 | Initial denaturation - 94°C/2 min 30 cycles: denaturation - 94°C/30 s annealing, - 50°C/30 s elongation - 72°C/1 min final elongation - 72°C/5 min |
| L2 CTC CAT AAA GGT GAC CCT | ||
| LM1 CCT AAG ACG CCA ATC GAA | 750 | |
| LM2 AAG CAC TTG CAA CTG CTC |
