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Western blot analysis of adalimumab from A. oryzae in the culture supernatant. The NSlD-ΔP10 transformant for producing adalimumab was cultured in 5 × DPY media, and culture supernatant was collected each day for analysis. a Time-course analysis in non-reducing condition and b the sample of day 6 in reducing condition. The arrow indicates the target IgG, and dashed arrows show the heavy chain and light chain around 50 kDa and 26 kDa, respectively
