. 2020 May 29;13:95. doi: 10.1186/s13068-020-01731-x

Table 2.

List of C4-oxidized XG oligosaccharides identified based on fragmentation patterns in CID-MS/MS present in the NcLPMO9C-TXG-digest

9C_C4-Ox Peak Nr.	Elution time (min)	m/z ([M−H]⁻)	MS/MS fragments (m/z)^a	Annotation
1	30.7–32.7	1059.4	293 (4), 453 (1), 527 (3), 605 (13), 765 (1), 821 (16), 839 (7), 899 (100), 999 (2), 1041 (14)	_O=GGXXX
1	30.7–32.7	1059.4	455 (1), 473 (1), 767 (4), 999 (2), 1041 (14)	_O=GXXXG
2	36.4–37.5	1221.5	311 (2), 767 (4), 1001 (5), 1061 (100), 1161 (2), 1203 (34)	_O=GGXLX/_O=GGXXL
			311 (2), 605 (3), 1001 (5), 1061 (100), 1161 (2), 1203 (34)	_O=GGLXX
			455 (8), 929 (12), 1161 (2), 1203 (39)	_O=GX(H₄P₂)
3	39.7–41.4	1383.7	311 (3), 1141 (1), 1163 (5), 1223 (100), 1365 (7)	_O=GG(H₅P₃)
4	43.0–45.0	1515.5	n.d.	_O=GH₆P₄

Chromatograms, including peak numbers, are shown in Fig. 5. Nomenclature (annotation) according to Fry et al. [15]

^aRelative intensities of MS/MS fragments are shown between brackets and informative MS/MS fragments are indicated in italics