Table 1.

Primers and probes used for Granada, Massilia and Arrabida detection

Name	Sequence (5′–3′)	Nucleotide positions
Primer_F	GAGCATGACATTAGCAGAGTTTCTGA	997–1022
Primer_Re	GTAGTCCCATTGCCAGCTTTCT	1102–1123
Ph_Probe	FAM-TGAGCTCTGATGAYATGTC-MGB-NFQ	1028–1046
IC_Probe	NED-CCAGCACACATGTGTCTACT-MGB-NFQ

Notes: Positions are based on the complete sequence from GR25 virus (GenBank: GU135606)

Abbreviations: MGB, minor groove binder; NFQ, non-fluorescent quencher