Table 4.
Phleboviruses genome used in this study to test the specificity of the assay
| Virus | Strain | GenBank ID | Origin | qRT-PCR result | Phlebo PCR result |
|---|---|---|---|---|---|
| Aguacate | VP 175 A | HM566138 | Culture | – | + |
| Anhanga | Be An 46852 | GU143711 | Culture | – | + |
| Arbia | ISS PHL18 | JX472400 | Culture | – | + |
| Arumowot | AR 1284-64 | GU143714 | Culture | – | + |
| Bujaru | Be An 47693 | GU143710 | Culture | – | + |
| Candiru | Be H22511 | GU143718 | Culture | – | + |
| Chagres | JW10 | GU143713 | Culture | – | + |
| Icoaraci | BeAn24262 | MK330768 | Culture | – | + |
| Karimabad | I-58 | GU143712 | Culture | – | + |
| Pacui | Be An 27326 | NC_043600 | Culture | – | + |
| Punique | DNA standard | – | |||
| Punta Toro | Balliet | KP272022 | Culture | – | + |
| Rift Valley | Saudi 200-10911 | DQ375401 | QC OMS | – | + |
| Rift Valley | Kenya 83 (21445) | DQ375402 | QC OMS | – | + |
| Rift Valley | 200803167 | JF311373 | QC OMS | – | + |
| Sand fly fever Sicilian | Sabin | EF095551 | Culture | – | + |
| Sand fly fever Naples | Sabin | Culture | – | + | |
| Toscana | Issh Phl.3 | Culture | – | + | |
| Toscana | sch | Culture | – | + |
Notes: Rift Valley genome was provided by an ENIVD quality control, quantified positive control of PUNV were specifically designed for this purpose, and the other phleboviruses belongs to the CNM virus collection