Fig. 9.

Exogenous proBDNF protein reduced CD4⁺ T cells but increased CD8⁺ T cells of septic mice in vitro. Mice injected with saline or LPS (5 mg kg⁻¹) for 5 days and the splenocytes were isolated and cultured for 3 days in vitro. Exogenous proBDNF did not alter the percentage of a CD3⁺ T cells in CD45⁺ cells or the percentage of b CD4⁺ T cells or c CD8⁺ T cells in CD3⁺ T cells in splenocytes from mice treated with saline. d Exogenous proBDNF did not alter the percentage of CD3⁺ T cells in CD45⁺ cells in splenocytes in septic mice. e–f ProBDNF treatment significantly decreased the percentage of e CD4⁺ T cells but increased the percentage of f CD8⁺ T cells in CD3⁺ T cells in splenocytes in LPS-treated mice. n = 4 in each group. Data were analyzed by one-way ANOVA and followed by Tukey post hoc test, *P < 0.05, **P < 0.01. Data are presented as mean ± SEM. NT = no treated control. proB = exogenous proBDNF protein treatment